FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001619921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2892557
Sequences flagged as poor quality0
Sequence length101
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC545051.8843189606980952Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAG414671.433575898417905TruSeq Adapter, Index 19 (97% over 38bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAG376141.3003719546408248TruSeq Adapter, Index 19 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA184510.637878527545006No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162900.5631695416892389No Hit
GTATACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAG132050.45651650079842854TruSeq Adapter, Index 19 (97% over 38bp)
ATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAG131340.4540619251409739TruSeq Adapter, Index 19 (97% over 38bp)
CATATGTATACATATGTATACATATGAGATCGGAAGAGCACACGTCTGAA128440.4440361935823564Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAA93490.32320884255694876TruSeq Adapter, Index 19 (97% over 38bp)
GTATACATATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAA88570.30619967039543217TruSeq Adapter, Index 19 (97% over 38bp)
ATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCT85040.29399593508442534TruSeq Adapter, Index 19 (97% over 38bp)
ATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA83920.2901239284135109TruSeq Adapter, Index 7 (97% over 37bp)
GTATACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCT80340.2777473356618383TruSeq Adapter, Index 19 (97% over 38bp)
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG66890.23124868412273294Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT54800.18945175496973785No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51650.17856173620779123No Hit
ACGTATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAG51410.17773202049259532TruSeq Adapter, Index 19 (97% over 38bp)
ATATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAG49310.1704720079846309TruSeq Adapter, Index 19 (97% over 38bp)
ATATGTATACATATGTATACATATAGATCGGAAGAGCACACGTCTGAACT48390.16729143107637984Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT48070.1661851434561186No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47850.16542457071718897No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT47110.16286628059533484No Hit
ACATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA44690.15449998046710922TruSeq Adapter, Index 7 (97% over 37bp)
GTATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA42210.14592625141008456TruSeq Adapter, Index 7 (97% over 37bp)
ACATATGTATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC40680.14063681372571052Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37170.12850222139096998No Hit
AGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34040.11768134560528971No Hit
TACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAA33650.11633305756809631TruSeq Adapter, Index 19 (97% over 38bp)
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33460.11567619929356622No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33160.1146390546495713No Hit
GTATACATATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAG30950.10699875577214207Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACATATGTATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAG30180.10433675118588846Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACATATGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAA29660.10253903380296396TruSeq Adapter, Index 19 (97% over 38bp)
CGTATATATACGTATATATACGAGATCGGAAGAGCACACGTCTGAACTCC29230.10105245981323789Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)