FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001619922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2892557
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG573201.9816376997929512Illumina Single End PCR Primer 1 (100% over 28bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT475161.6426988301354128No Hit
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT416051.4383467637802816Illumina Single End PCR Primer 1 (97% over 35bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT380051.313889406500892Illumina Single End PCR Primer 1 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA359601.2431907132685718No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT188870.6529516963710654No Hit
CATATGTATACATATGTATACATATGAGATCGGAAGAGCGTCGTGTAGGG137650.4758765341530003Illumina Single End PCR Primer 1 (100% over 24bp)
GTATACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT133730.4623245108048Illumina Single End PCR Primer 1 (97% over 35bp)
ATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT131880.4559287855001647Illumina Single End PCR Primer 1 (97% over 35bp)
GTATACATATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTA93730.32403855827214467Illumina Single End PCR Primer 1 (97% over 35bp)
CATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTA89670.31000253409008016Illumina Single End PCR Primer 1 (97% over 35bp)
GTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAG86400.2986976574705356Illumina Single End PCR Primer 1 (97% over 35bp)
ATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCT85200.29454907889455595Illumina Single End PCR Primer 1 (97% over 35bp)
ATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAG80630.27874990881770007Illumina Single End PCR Primer 1 (97% over 35bp)
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG78670.27197389714359993Illumina Single End PCR Primer 1 (100% over 30bp)
ATATGTATACATATGTATACATATAGATCGGAAGAGCGTCGTGTAGGGAA56500.1953289079523757Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATACGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT51260.17721344817059786Illumina Single End PCR Primer 1 (97% over 35bp)
ACGTATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT50020.17292658364208555Illumina Single End PCR Primer 1 (97% over 35bp)
GTATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCT44640.15432712302644339Illumina Single End PCR Primer 1 (97% over 35bp)
ACATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCT42510.1469633960540795Illumina Single End PCR Primer 1 (97% over 35bp)
ACATATGTATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG41310.14281481747809982Illumina Single End PCR Primer 1 (100% over 32bp)
ATACATATGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTA34200.11823448941542032Illumina Single End PCR Primer 1 (97% over 35bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA32160.11118190583625492No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA31570.10914218803639825No Hit
ATACATATGTATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAG31460.10876190166693345Illumina Single End PCR Primer 1 (100% over 30bp)
GTATACATATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG30770.10637646898574514Illumina Single End PCR Primer 1 (100% over 30bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTAAA30620.10585789666374767No Hit
TACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTA30190.10437132267402163Illumina Single End PCR Primer 1 (97% over 35bp)
ATACATATGTATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTT29830.10312674910122772No Hit
CATATGTATACATATGTATACATATGTATACATATGAGATCGGAAGAGCG29780.1029538916605619No Hit
CGTATATATACGTATATATACGAGATCGGAAGAGCGTCGTGTAGGGAAAG29590.10229703338603181Illumina Single End PCR Primer 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)