FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001619929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118816
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA608235.436372021851672No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227842.036438520721906No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT156901.402375368246432No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147481.3181792180304894No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107990.965216800617796No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105270.9409053856934474No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95010.8492012985155737No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93980.8399951377170152No Hit
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC91980.8221190973314647Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT88670.7925342504933788No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT76260.6816134199010383No Hit
CGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73460.6565869633612677No Hit
CGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72560.64854274518777No Hit
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGG71620.6401410062065612TruSeq Adapter, Index 7 (97% over 36bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGG65260.5832951977805108TruSeq Adapter, Index 7 (97% over 36bp)
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59450.5313653004604868No Hit
ATATGGATCCGGCGCGCCCTCGAGATATAGATCGGAAGAGCACACGTCTG53810.48095486657323455Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATGGATCCGGCGCGCCGTCGAGATATAGATCGGAAGAGCACACGTCTG50640.4526213425621371Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA43980.39309412807825417Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42450.3794189571833081No Hit
ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36690.3279359608729228No Hit
CGAGGGCGCGCCGGATCCATATAGATCGGAAGAGCACACGTCTGAACTCC34340.30693161341990105Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33310.29772545262134253No Hit
GCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32790.29307768212109947No Hit
ATCTCGAGGGCGCGCCGGATCCATATAGATCGGAAGAGCACACGTCTGAA30750.274844120927838Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28350.2533928724651775No Hit
GATCCGGCGCGCCCTCGAGATATAGATCGGAAGAGCACACGTCTGAACTC28180.2518734090324057Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27690.24749377913794585No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27630.24695749792637933No Hit
CATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTG27340.2443654720704745TruSeq Adapter, Index 7 (97% over 36bp)
AAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC25740.23006463976203415Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24270.21692575007865458No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT23810.212814260789978No Hit
GGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23420.20932843291479564No Hit
GTATACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGG22560.20164173554900897TruSeq Adapter, Index 7 (97% over 36bp)
CTCGAGGGCGCGCCGGATCCATATAGATCGGAAGAGCACACGTCTGAACT22480.20092669393358695Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTATACATATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTG22240.1987815690873209TruSeq Adapter, Index 7 (97% over 36bp)
GATCCGGCGCGCCGTCGAGATATAGATCGGAAGAGCACACGTCTGAACTC22210.1985134284815376Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22200.19842404827960985No Hit
TTTTTTTTTTTTTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA22100.19753024626033236Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGG21590.192971855962017TruSeq Adapter, Index 7 (97% over 36bp)
GGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21570.1927930955581615No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT21370.19100549151960644No Hit
AAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG21120.18877098647141263Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT20360.18197809112490348TruSeq Adapter, Index 7 (97% over 36bp)
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19590.17509581557646653No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19220.17178874810513972No Hit
GAGGGCGCGCCGGATCCATATAGATCGGAAGAGCACACGTCTGAACTCCA17630.15757729599862713Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17030.152214483882962No Hit
ATGGATCCGGCGCGCCCTCGAGATATAGATCGGAAGAGCACACGTCTGAA16660.14890741641163516Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGATCCGGCGCGCCCTCGAGATATAGATCGGAAGAGCACACGTCTGAACT16460.1471198123730801Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGT16450.14703043217115236TruSeq Adapter, Index 7 (97% over 36bp)
GGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16040.14336584389211454No Hit
TCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15170.13558976632440006No Hit
TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15150.13541100592054459No Hit
ATCCGGCGCGCCGTCGAGATATAGATCGGAAGAGCACACGTCTGAACTCC14320.12799244916054114Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC13990.12504290249692532Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13970.12486414209306983No Hit
TCTCGAGGGCGCGCCGGATCCATATAGATCGGAAGAGCACACGTCTGAAC13900.12423848067957555Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTTTTTTTTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC13860.12388095987186454Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13440.12012699139089895No Hit
ATCCGGCGCGCCCTCGAGATATAGATCGGAAGAGCACACGTCTGAACTCC13260.11851814775619941Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGT13060.11673054371764437TruSeq Adapter, Index 7 (97% over 36bp)
GTATACATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGT13010.1162836427080056TruSeq Adapter, Index 7 (97% over 36bp)
ACATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGT12980.11601550210222233TruSeq Adapter, Index 7 (97% over 36bp)
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAA12960.11583674169836686No Hit
ATATCTCGAGGGCGCGCCGGATCCATATAGATCGGAAGAGCACACGTCTG12870.11503231988101707Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12690.11342347624631753No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12680.11333409604438978No Hit
ATATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGT12620.11279781483282326TruSeq Adapter, Index 7 (97% over 36bp)
TTTTTTTTTTTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG12440.11118897119812372Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12410.11092083059234047No Hit
GCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11490.10269785201498727No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)