FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001619930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118816
Sequences flagged as poor quality0
Sequence length151
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT776496.94028329948803No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA395693.5366852100792268No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT163831.4643158481823642No Hit
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG105260.9408160054915196Illumina Single End PCR Primer 1 (100% over 28bp)
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC67030.599115493521723Illumina Single End PCR Primer 1 (100% over 40bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC63300.5657766782026714Illumina Single End PCR Primer 1 (100% over 40bp)
ATATCTCGAGGGCGCGCCGGATCCATATAGATCGGAAGAGCGTCGTGTAG51590.4611124617452736Illumina Single End PCR Primer 1 (100% over 22bp)
ATATCTCGACGGCGCGCCGGATCCATATAGATCGGAAGAGCGTCGTGTAG48720.43546034379200865Illumina Single End PCR Primer 1 (100% over 22bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46270.4135621943197094No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA35490.31721033664159254No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTAAA34420.307646655035323No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31500.28154763607241945No Hit
ATATGGATCCGGCGCGCCCTCGAGATCGGAAGAGCGTCGTGTAGGGAAAG31020.2772573863798873Illumina Single End PCR Primer 1 (100% over 28bp)
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT30260.27046449103337816No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28380.25366101307096073No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28290.252856591253611No Hit
ATATGGATCCGGCGCGCCCTCGAGATAGATCGGAAGAGCGTCGTGTAGGG27990.25017518519577836Illumina Single End PCR Primer 1 (100% over 24bp)
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27020.24150530560878644No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26980.2411477848010754No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26030.23265666561793893No Hit
GTATACATATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT25710.2297964991562509Illumina Single End PCR Primer 1 (100% over 39bp)
ATATCTCGAGGGCGCGCCGGATCAGATCGGAAGAGCGTCGTGTAGGGAAA25320.22631067128106855Illumina Single End PCR Primer 1 (100% over 27bp)
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24780.22148414037696995No Hit
CATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT21700.19395503818322227Illumina Single End PCR Primer 1 (100% over 39bp)
ATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC21520.19234619454852273Illumina Single End PCR Primer 1 (100% over 40bp)
GTATACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC21060.18823470525984612Illumina Single End PCR Primer 1 (100% over 40bp)
ATATGGATCCGGCGCGCCCTCGAGAGATCGGAAGAGCGTCGTGTAGGGAA20540.183586934759603Illumina Single End PCR Primer 1 (100% over 26bp)
ATATCTCGACGGCGCGCCGGATCAGATCGGAAGAGCGTCGTGTAGGGAAA20100.1796542058747819Illumina Single End PCR Primer 1 (100% over 27bp)
AAAAAAAAAAAAAAAAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT19650.17563209678803307Illumina Single End PCR Primer 1 (100% over 33bp)
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19580.1750064353745388No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA19330.172771930326345No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18510.1654427537682693No Hit
TTTTTTTTTTTTTTTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT17410.15561093155621658Illumina Single End PCR Primer 1 (100% over 33bp)
ATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC17120.15301890570031176Illumina Single End PCR Primer 1 (100% over 38bp)
ATATGGATCCGGCGCGCCCTCAGATCGGAAGAGCGTCGTGTAGGGAAAGA16150.1443490261133198Illumina Single End PCR Primer 1 (100% over 29bp)
ATATCTCGAGGGCGCGCCGGATCCATAGATCGGAAGAGCGTCGTGTAGGG16130.14417026570946428Illumina Single End PCR Primer 1 (100% over 24bp)
ATATCTCGAGGGCGCGCCGGATCCAGATCGGAAGAGCGTCGTGTAGGGAA15320.13693046935331638Illumina Single End PCR Primer 1 (100% over 26bp)
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14650.13094199582415697No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13390.11968009038126018No Hit
AAAAAAAAAAAAAAAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA13170.11771372593884963Illumina Single End PCR Primer 1 (100% over 34bp)
ATATCTCGACGGCGCGCCGGATAGATCGGAAGAGCGTCGTGTAGGGAAAG13090.11699868432342762Illumina Single End PCR Primer 1 (100% over 28bp)
ATATGGATCCGGCGCGCCCTCGAGAAGATCGGAAGAGCGTCGTGTAGGGA12980.11601550210222233Illumina Single End PCR Primer 1 (100% over 25bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTAAAA12480.11154649200583475No Hit
ATATGGATCCGGCGCGCCCTCGAGATATAGATCGGAAGAGCGTCGTGTAG12480.11154649200583475Illumina Single End PCR Primer 1 (100% over 22bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCT12340.1102951691788462No Hit
ACATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC12330.11020578897691846Illumina Single End PCR Primer 1 (100% over 38bp)
ATATCTCGAGGGCGCGCCGGATAGATCGGAAGAGCGTCGTGTAGGGAAAG12230.10931198695764094Illumina Single End PCR Primer 1 (100% over 28bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12140.10850756514029117No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12140.10850756514029117No Hit
GTATACATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC12070.1078819037267969Illumina Single End PCR Primer 1 (100% over 38bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA12050.1077031433229414No Hit
ATATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC12030.10752438291908588Illumina Single End PCR Primer 1 (100% over 38bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC12020.10743500271715814No Hit
GTATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC11780.1052898778708921Illumina Single End PCR Primer 1 (100% over 38bp)
CGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11720.10475359665932558No Hit
TTTTTTTTTTTTTTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA11660.10421731544775906Illumina Single End PCR Primer 1 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)