FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001619933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686484
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC739524.3849808240101895Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAAT323701.9193778298519288TruSeq Adapter, Index 9 (97% over 36bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAAT302091.7912414229841491TruSeq Adapter, Index 9 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA241971.4347601281719837No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209941.2448383738001665No Hit
ATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATA157980.9367417657090136TruSeq Adapter, Index 9 (97% over 36bp)
ATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAAT141260.8376005938983115TruSeq Adapter, Index 9 (97% over 36bp)
GTATACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAAT137760.8168473581723871TruSeq Adapter, Index 9 (97% over 36bp)
CATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAA83780.4967731683194148TruSeq Adapter, Index 9 (97% over 36bp)
GTATACATATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAA75830.44963367574195784TruSeq Adapter, Index 9 (97% over 36bp)
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG68100.4037986722672732Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATATGTATACATATGTATACATATGAGATCGGAAGAGCACACGTCTGAA61920.36715438747121226Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CATATGTATACATATGTATACATATGTATACATATGAGATCGGAAGAGCA57610.34159826004871674No Hit
ACATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATA56930.3375662028219657TruSeq Adapter, Index 9 (97% over 36bp)
GTATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATA56120.3327633111253946TruSeq Adapter, Index 9 (97% over 36bp)
CGTATATATACGTATATATACGTATATATACGAGATCGGAAGAGCACACG50970.3022264071286772No Hit
ATATACGTATATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCC49430.2930949834092704Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGTATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAAT41570.24648914546476575TruSeq Adapter, Index 9 (97% over 36bp)
ATATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAAT41540.24631126058711497TruSeq Adapter, Index 9 (97% over 36bp)
ATACATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA40260.2387215058073483TruSeq Adapter, Index 7 (97% over 35bp)
GTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA39470.23403720402921108TruSeq Adapter, Index 7 (97% over 35bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT37280.22105160796070406No Hit
ATACATATATGTATACATATATGTATAGATCGGAAGAGCACACGTCTGAA37240.22081442812383634Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT32220.19104835859693894No Hit
ATACGTATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATA30390.1801973810602413TruSeq Adapter, Index 9 (97% over 36bp)
ATATATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATA29410.17438647505698246TruSeq Adapter, Index 9 (97% over 36bp)
ATATGTATACATATGTATACATATAGATCGGAAGAGCACACGTCTGAACT29010.17201467668830536Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28710.17023582791179756No Hit
ACATATGTATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC28610.1696428783196283Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27050.16039286468178768No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26450.15683516712877205No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24750.14675502406189445No Hit
ATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATAT23350.13845372977152468TruSeq Adapter, Index 9 (97% over 36bp)
GTATACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATAT22950.13608193140284758TruSeq Adapter, Index 9 (97% over 36bp)
ATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAAT20270.12019088233271114TruSeq Adapter, Index 9 (97% over 36bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19310.11449856624788614No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17950.10643445179438407No Hit
GTATATATACGTATATATACGTATATATACAGATCGGAAGAGCACACGTC17710.10501137277317782No Hit
ATATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATA17610.10441842318100854TruSeq Adapter, Index 9 (97% over 36bp)
CATATGTATACATATGTATACATATGTATACATATGTATACATATGTATA17250.10228380464919916No Hit
GTATACATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATA17060.10115720042407755TruSeq Adapter, Index 9 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)