FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001620473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001620473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524739
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC46810.8920625301340286Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATATGTACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG21030.40077066884679813Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG9030.17208555110254814Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGA5450.10386115764218021TruSeq Adapter, Index 6 (97% over 36bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGA5400.10290830298491249TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)