FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001620474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001620474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524739
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG49140.9364655571627036Illumina Single End PCR Primer 1 (100% over 28bp)
ACATATATGTACATATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG21400.40782179331057916Illumina Single End PCR Primer 1 (100% over 30bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT18350.34969765921724894No Hit
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG10510.2002900489576723Illumina Single End PCR Primer 1 (100% over 30bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT8970.17094212551382687No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6830.13015994618276897No Hit
ATATGTATACATATGTATACATATAGATCGGAAGAGCGTCGTGTAGGGAA6050.1152954135293927Illumina Single End PCR Primer 1 (100% over 26bp)
ATATGTACATATATGTACATATAGATCGGAAGAGCGTCGTGTAGGGAAAG5620.1071008634768904Illumina Single End PCR Primer 1 (100% over 28bp)
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG5570.1061480088196227Illumina Single End PCR Primer 1 (97% over 35bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG5470.1042422995050873Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)