FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001620491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001620491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135400
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCG122461.078562621102695TruSeq Adapter, Index 6 (97% over 36bp)
ATATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCG118611.0446538664787741TruSeq Adapter, Index 6 (97% over 36bp)
GTATATATACGTATATATACAGATCGGAAGAGCACACGTCTGAACTCCAG113611.000616522811344Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATACGTATATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCC84840.7472256473489519Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCG33710.29689977100581294TruSeq Adapter, Index 6 (97% over 36bp)
ATATATACGTATATATACGTATATATAGATCGGAAGAGCACACGTCTGAA31350.27611414479478597Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCG31340.2760260701074511TruSeq Adapter, Index 6 (97% over 36bp)
ATATATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG31060.27355997886207506TruSeq Adapter, Index 6 (97% over 36bp)
ATACGTATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG30960.2726792319887264TruSeq Adapter, Index 6 (97% over 36bp)
TACGTATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGC15450.13607539193235862TruSeq Adapter, Index 6 (97% over 36bp)
ATATATACGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGC15090.1329047031883037TruSeq Adapter, Index 6 (97% over 36bp)
CGTATATATACGTATATATACGAGATCGGAAGAGCACACGTCTGAACTCC14660.1291174916329047Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT14510.1277963713228818No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT13520.11907697727673067No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT12750.11229522635194644No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)