FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001620492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001620492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135400
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATACGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG122301.0771534261053373Illumina Single End PCR Primer 1 (97% over 35bp)
ACGTATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG119811.0552228289589571Illumina Single End PCR Primer 1 (97% over 35bp)
GTATATATACGTATATATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAG114951.0124185309142153Illumina Single End PCR Primer 1 (100% over 30bp)
ATATACGTATATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAG93780.8259644178263167Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATACGTATATATACGTATATATAGATCGGAAGAGCGTCGTGTAGGG33930.29883741412717985Illumina Single End PCR Primer 1 (100% over 24bp)
ATATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG33830.2979566672538313Illumina Single End PCR Primer 1 (97% over 35bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT33020.2908226175797076No Hit
ATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG31620.2784921613528272Illumina Single End PCR Primer 1 (97% over 35bp)
ATACGTATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT31440.2769068169807997Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT31130.27417650167341906Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATACGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATA15380.13545886912101462Illumina Single End PCR Primer 1 (97% over 35bp)
TACGTATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATA15260.1344019728729963Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATATATACGTATATATACGAGATCGGAAGAGCGTCGTGTAGGGAAAG14830.13061476131759733Illumina Single End PCR Primer 1 (100% over 28bp)
CGTATATATACGTATATATACGTATATATACGAGATCGGAAGAGCGTCGT12840.1130878985379602No Hit
ATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT11400.10040514356174036Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)