FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001620493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001620493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838896
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC714303.884395854904247Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT272561.4821936640245015TruSeq Adapter, Index 27 (97% over 39bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT248871.353366367646675TruSeq Adapter, Index 27 (97% over 39bp)
ACATATATGTACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG177450.9649811626106098Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120270.6540337245825757No Hit
ATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT110610.6015022056712288TruSeq Adapter, Index 27 (97% over 39bp)
GTATACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT106230.5776835666617363TruSeq Adapter, Index 27 (97% over 39bp)
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG76350.41519476903533425Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCG75320.40959358223629827TruSeq Adapter, Index 27 (97% over 39bp)
GTATACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCG72070.3919199345694373TruSeq Adapter, Index 27 (97% over 39bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63740.3466210160879136No Hit
CATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC63520.345424646092003TruSeq Adapter, Index 1 (97% over 38bp)
GTATACATATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC55790.30338855487205363TruSeq Adapter, Index 1 (97% over 38bp)
ATATACATATATATGTATATAGATCGGAAGAGCACACGTCTGAACTCCAG50200.27298988088505277Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATGTACATATATGTACATATAGATCGGAAGAGCACACGTCTGAACTCC49910.27141284770862517Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA48630.2644521495505999TruSeq Adapter, Index 1 (97% over 37bp)
GTACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCG48260.2624400727392957TruSeq Adapter, Index 27 (97% over 39bp)
ATATGTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCG45100.24525584916167092TruSeq Adapter, Index 27 (97% over 39bp)
ATATATGTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT44350.241177315084703TruSeq Adapter, Index 27 (97% over 39bp)
ATATGTACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT42860.23307462738512674TruSeq Adapter, Index 27 (97% over 39bp)
ATGTACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT41610.22627707059018018TruSeq Adapter, Index 27 (97% over 39bp)
ATATGTATACATATGTATACATATAGATCGGAAGAGCACACGTCTGAACT41080.22339490650912286Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTACATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT40160.21839190470804223TruSeq Adapter, Index 27 (97% over 39bp)
ACATATGTATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC31480.17118967032393348Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT26670.14503267177697923TruSeq Adapter, Index 27 (97% over 39bp)
ACGTATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT26390.1435100190549112TruSeq Adapter, Index 27 (97% over 39bp)
TACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC25540.13888768043434754TruSeq Adapter, Index 1 (97% over 38bp)
ATATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA25210.1370931254404817TruSeq Adapter, Index 1 (97% over 37bp)
ACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT24880.1352985704466158TruSeq Adapter, Index 27 (97% over 39bp)
ATACATATATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC24260.1319269822763223Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA24250.13187260182196275TruSeq Adapter, Index 1 (97% over 37bp)
ATACATATGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC24080.13094813409785TruSeq Adapter, Index 1 (97% over 38bp)
GTATACATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA23780.12931672046706286TruSeq Adapter, Index 1 (97% over 37bp)
CATATATGTACATATATGTACATATATGAGATCGGAAGAGCACACGTCTG23170.12599951275112894Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA22990.12502066457265665TruSeq Adapter, Index 1 (97% over 37bp)
ATATATATGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT22870.12436809912034177TruSeq Adapter, Index 1 (100% over 35bp)
GTATATATACGTATATATACAGATCGGAAGAGCACACGTCTGAACTCCAG21650.11773368368847396Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATACGTATATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCC21400.11637417232948466Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATGTATATACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC21020.11430771506382091Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)