FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001620494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001620494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838896
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG751244.085277253308507Illumina Single End PCR Primer 1 (100% over 28bp)
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG275061.4957887776143948Illumina Single End PCR Primer 1 (97% over 35bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG253171.376749963021291Illumina Single End PCR Primer 1 (97% over 35bp)
ACATATATGTACATATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG180680.9825460493687518Illumina Single End PCR Primer 1 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA156370.8503471648206316No Hit
GTATACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG112610.6123782965431434Illumina Single End PCR Primer 1 (97% over 35bp)
ATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG107640.5853512107264359Illumina Single End PCR Primer 1 (97% over 35bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT96380.5241188191175575No Hit
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG90680.4931219601326013Illumina Single End PCR Primer 1 (100% over 30bp)
GTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGG77210.4198714881102575Illumina Single End PCR Primer 1 (97% over 35bp)
ATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGG72770.39572656637460735Illumina Single End PCR Primer 1 (97% over 35bp)
GTATACATATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATA63690.3463491138161157Illumina Single End PCR Primer 1 (97% over 35bp)
ATATGTACATATATGTACATATAGATCGGAAGAGCGTCGTGTAGGGAAAG63260.34401075427865413Illumina Single End PCR Primer 1 (100% over 28bp)
CATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATA57160.31083867711931507Illumina Single End PCR Primer 1 (97% over 35bp)
ATATACATATATATGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG51790.28163637312822476Illumina Single End PCR Primer 1 (100% over 30bp)
ATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT49580.2696182927147593Illumina Single End PCR Primer 1 (97% over 35bp)
ATATGTATACATATGTATACATATAGATCGGAAGAGCGTCGTGTAGGGAA48860.2657029000008701Illumina Single End PCR Primer 1 (100% over 26bp)
ATATGTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGG48720.26494157363983606Illumina Single End PCR Primer 1 (97% over 35bp)
GTACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGG46400.2523253082284153Illumina Single End PCR Primer 1 (97% over 35bp)
GTACATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG45280.24623469734014322Illumina Single End PCR Primer 1 (97% over 35bp)
ATGTACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG43680.23753382464261164Illumina Single End PCR Primer 1 (97% over 35bp)
ATATGTACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG42110.22899609330815884Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATGTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG40790.2218178733326953Illumina Single End PCR Primer 1 (97% over 35bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT37270.20267595339812586No Hit
ACATATGTATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG32090.17450687803986742Illumina Single End PCR Primer 1 (100% over 32bp)
ACGTATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG27070.14720788995136214Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATACGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG26410.14361877996363037Illumina Single End PCR Primer 1 (97% over 35bp)
ATACATATGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATA26120.14204174678720274Illumina Single End PCR Primer 1 (97% over 35bp)
GTATACATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT25640.13943148497794328Illumina Single End PCR Primer 1 (97% over 35bp)
ACATATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG25330.13774569089279654Illumina Single End PCR Primer 1 (97% over 35bp)
CATATATGTACATATATGTACATATATGAGATCGGAAGAGCGTCGTGTAG25170.13687560362304338Illumina Single End PCR Primer 1 (100% over 22bp)
ATACATATATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA24940.13562485317277323Illumina Single End PCR Primer 1 (100% over 34bp)
GTATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT24540.13344963499839033Illumina Single End PCR Primer 1 (97% over 35bp)
TACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATA24530.13339525454403078Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT24090.1310025145522096Illumina Single End PCR Primer 1 (97% over 35bp)
ATATACGTATATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAG23700.12888167683218626Illumina Single End PCR Primer 1 (100% over 28bp)
ACATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT23400.1272502632013991Illumina Single End PCR Primer 1 (97% over 35bp)
ATATACATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGG23040.1252925668444545Illumina Single End PCR Primer 1 (97% over 35bp)
GTATATATACGTATATATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAG21930.11925633641054198Illumina Single End PCR Primer 1 (100% over 30bp)
ATATGTATATACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA21710.1180599664146314Illumina Single End PCR Primer 1 (100% over 34bp)
CATATGTATACATATGTATACATATGAGATCGGAAGAGCGTCGTGTAGGG19680.10702073417963821Illumina Single End PCR Primer 1 (100% over 24bp)
ATGTACATATATGTACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG18730.10185459101547886Illumina Single End PCR Primer 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)