FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001620495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001620495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199516
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33890.28253062068367574No Hit
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC29950.24968403922915575Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT21840.18207343628596867No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT21320.1777383544696361No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT15890.1324700962721631No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)