FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001621025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001621025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143497
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC152461.333278530682634Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT104840.9168366860603918TruSeq Adapter, Index 27 (97% over 39bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT96010.8396174191974269TruSeq Adapter, Index 27 (97% over 39bp)
ATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT24910.2178405365296105TruSeq Adapter, Index 27 (97% over 39bp)
GTATACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT23630.2066468036208228TruSeq Adapter, Index 27 (97% over 39bp)
CATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC21750.19020600841104088TruSeq Adapter, Index 1 (97% over 38bp)
ATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCG21510.18810718349064318TruSeq Adapter, Index 27 (97% over 39bp)
ATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA20210.17673854850515566TruSeq Adapter, Index 1 (97% over 37bp)
GTATACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCG19550.17096677997406204TruSeq Adapter, Index 27 (97% over 39bp)
GTATACATATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC18420.1610848126405229TruSeq Adapter, Index 1 (97% over 38bp)
TACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC14020.1226063557665652TruSeq Adapter, Index 1 (97% over 38bp)
ATACATATGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC13440.11753419554227076TruSeq Adapter, Index 1 (97% over 38bp)
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG12970.11342399673982528Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)