FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001621026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001621026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143497
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG159631.395980925179515Illumina Single End PCR Primer 1 (100% over 28bp)
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTA98610.8623546891684017Illumina Single End PCR Primer 1 (96% over 33bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTA91100.796678959367624Illumina Single End PCR Primer 1 (96% over 33bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT48950.42807283272277935No Hit
GTATACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTA23860.20865817750287058Illumina Single End PCR Primer 1 (96% over 33bp)
ATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTA22170.19387895202173683Illumina Single End PCR Primer 1 (96% over 33bp)
GTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATG20320.17770050992700462Illumina Single End PCR Primer 1 (96% over 33bp)
GTATACATATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCT20250.1770883526585553Illumina Single End PCR Primer 1 (96% over 33bp)
ATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTC19350.16921775920706394Illumina Single End PCR Primer 1 (96% over 33bp)
ATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATG18340.16038520433372366Illumina Single End PCR Primer 1 (96% over 33bp)
CATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCT17460.15268951295893213Illumina Single End PCR Primer 1 (96% over 33bp)
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG14910.13038949817970663Illumina Single End PCR Primer 1 (100% over 30bp)
ATACATATGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCT13510.1181463528107201Illumina Single End PCR Primer 1 (96% over 33bp)
TACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCT12950.11324909466312548Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)