FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001621033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001621033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832933
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23640.28381634537231687No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT20340.24419731238887163No Hit
ACATATATGTACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG12960.15559474771680315Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12360.14839128717435854No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9600.11525536867911346No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT8530.10240919737842061No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)