FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001621038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001621038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352420
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT128320.9488176749826237No Hit
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG105730.781783765398323Illumina Single End PCR Primer 1 (100% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64520.4770707324647669No Hit
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTA25410.18788542020969817Illumina Single End PCR Primer 1 (96% over 33bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT24240.17923426154596944No Hit
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTA24240.17923426154596944Illumina Single End PCR Primer 1 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)