FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001621039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001621039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187172
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC97160.818415528668129Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGC41970.353529227441348TruSeq Adapter, Index 8 (97% over 36bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGC38900.3276694531205251TruSeq Adapter, Index 8 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38210.3218573214327831No Hit
ACATATATGTACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG34510.29069081817967407Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23140.19491699602079565No Hit
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG12690.10689268277890651Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)