FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001621040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001621040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187172
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG101160.8521090456985172Illumina Single End PCR Primer 1 (100% over 28bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT65310.5501308993136631No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63330.5334526083836209No Hit
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTA39070.3291014275943166Illumina Single End PCR Primer 1 (96% over 33bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTA36270.30551596567304484Illumina Single End PCR Primer 1 (96% over 33bp)
ACATATATGTACATATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG34820.29330206574952916Illumina Single End PCR Primer 1 (100% over 30bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT16520.1391542253355032No Hit
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG14290.12037008959106178Illumina Single End PCR Primer 1 (100% over 30bp)
ATATGTACATATATGTACATATAGATCGGAAGAGCGTCGTGTAGGGAAAG11910.10032244695798083Illumina Single End PCR Primer 1 (100% over 28bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)