FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001621579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001621579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833104
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA177472.1302262382607693No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91091.093380898423246No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT17740.21293860070291343No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT16780.20141542952620564No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT15890.19073248958113273No Hit
ACATATATGTACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG14250.17104707215425685Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12180.1462002343044806No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11750.1410388138815802No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11500.13803798805431255No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10800.12963567573796309No Hit
ATATGGATCCGGCGCGCCGTCGAGATATAGATCGGAAGAGCACACGTCTG9320.11187078684053851Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8720.10466880485509612No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)