FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001621583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001621583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297387
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC250081.9275667167930617Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGC149671.153626481535579TruSeq Adapter, Index 3 (97% over 36bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGC130491.0057908704187724TruSeq Adapter, Index 3 (97% over 36bp)
ATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGC60040.4627763342780527TruSeq Adapter, Index 3 (97% over 36bp)
GTATACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGC58170.44836274758418265TruSeq Adapter, Index 3 (97% over 36bp)
ATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGC39790.30669337676421915TruSeq Adapter, Index 3 (97% over 36bp)
GTATACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGC38380.2958253782410337TruSeq Adapter, Index 3 (97% over 36bp)
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG26980.2079564540110237Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG26900.20733982998133943TruSeq Adapter, Index 3 (97% over 36bp)
GTATACATATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG23810.18352272683478407TruSeq Adapter, Index 3 (97% over 36bp)
ATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAT23330.1798229826566784TruSeq Adapter, Index 3 (97% over 36bp)
ACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGC21780.16787589208154546TruSeq Adapter, Index 3 (97% over 36bp)
ATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGC19360.14922301518359596TruSeq Adapter, Index 3 (97% over 36bp)
ACATATATGTACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG18830.14513788098693758Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGC17520.1350406625008575TruSeq Adapter, Index 3 (97% over 36bp)
TACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG16160.12455805399622473TruSeq Adapter, Index 3 (97% over 36bp)
ATACATATGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG15200.11715856564001334TruSeq Adapter, Index 3 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)