FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001621584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001621584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297387
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG260162.0052613445332814Illumina Single End PCR Primer 1 (100% over 28bp)
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA134731.0384719439920393Illumina Single End PCR Primer 1 (96% over 33bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA120340.9275566966525794Illumina Single End PCR Primer 1 (96% over 33bp)
GTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACG57180.44073202521683963Illumina Single End PCR Primer 1 (96% over 33bp)
ATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACG54610.4209229782632322Illumina Single End PCR Primer 1 (96% over 33bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT41820.3223402115174578No Hit
GTATACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA37160.28642186178834844Illumina Single End PCR Primer 1 (96% over 33bp)
ATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA34490.2658420347976356Illumina Single End PCR Primer 1 (96% over 33bp)
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG31160.2401750595620274Illumina Single End PCR Primer 1 (100% over 30bp)
GTATACATATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT23610.18198116676057335Illumina Single End PCR Primer 1 (96% over 33bp)
ATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAG22820.17589200446744108Illumina Single End PCR Primer 1 (96% over 33bp)
CATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT21550.16610309799620315Illumina Single End PCR Primer 1 (96% over 33bp)
ATATACGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACG20100.15492678745817556Illumina Single End PCR Primer 1 (96% over 33bp)
ACATATATGTACATATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG18950.146062817031464Illumina Single End PCR Primer 1 (100% over 30bp)
ACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACG18590.14328800889788473Illumina Single End PCR Primer 1 (96% over 33bp)
ATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACG16950.130647216289357Illumina Single End PCR Primer 1 (96% over 33bp)
ATACATATGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT15150.11677317562146068Illumina Single End PCR Primer 1 (96% over 33bp)
TACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT14060.10837167321701235Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)