FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001621585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001621585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042172
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATATATGTACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG132861.2748375508073524Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102650.9849621751495914No Hit
ATATGTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAA47420.45501126493515465TruSeq Adapter, Index 13 (97% over 38bp)
GTACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAA47060.45155694069692914TruSeq Adapter, Index 13 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39210.3762334816133997No Hit
ATATGTACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAG38880.37306701772835965TruSeq Adapter, Index 13 (97% over 38bp)
ATGTACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAG36250.3478312600991007TruSeq Adapter, Index 13 (97% over 38bp)
ATATGTACATATATGTACATATAGATCGGAAGAGCACACGTCTGAACTCC34080.32700936121868557Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAG24530.23537381545464664TruSeq Adapter, Index 13 (97% over 38bp)
GTACATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAG23820.22856112042925736TruSeq Adapter, Index 13 (97% over 38bp)
ATATATGTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAG23530.22577847034846457TruSeq Adapter, Index 13 (97% over 38bp)
CATATATGTACATATATGTACATATATGAGATCGGAAGAGCACACGTCTG13910.13347125042699287Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT11960.11476032746993779No Hit
CATATATGTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA10850.10410949440207568TruSeq Adapter, Index 13 (97% over 38bp)
GTATATATACGTATATATACAGATCGGAAGAGCACACGTCTGAACTCCAG10750.10314995989145746Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)