FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001621587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001621587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1151674
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC98100.8518035485736415Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATATGTACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG48430.42051830639573357Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTC21390.1857296422425096TruSeq Adapter, Index 7 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20270.17600466798764233No Hit
GTACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGT18680.16219867775082186TruSeq Adapter, Index 7 (97% over 35bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTC18470.16037524507803422TruSeq Adapter, Index 7 (97% over 35bp)
ATATGTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGT17780.1543839662960178TruSeq Adapter, Index 7 (97% over 35bp)
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG15240.13232911396801525Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT12140.10541177451257908No Hit
ATATGTACATATATGTACATATAGATCGGAAGAGCACACGTCTGAACTCC12040.10454347323982309Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)