FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001621588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001621588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1151674
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG102520.890182464829457Illumina Single End PCR Primer 1 (100% over 28bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT75180.652788896857965No Hit
ACATATATGTACATATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG48980.42529396339589154Illumina Single End PCR Primer 1 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28670.2489419748991468No Hit
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCT19010.16506407195091669Illumina Single End PCR Primer 1 (97% over 34bp)
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG17380.15091076120499378Illumina Single End PCR Primer 1 (100% over 30bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCT16880.14656925484121375Illumina Single End PCR Primer 1 (97% over 34bp)
ATATGTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGG16650.14457216191387492Illumina Single End PCR Primer 1 (97% over 34bp)
GTACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGG16340.14188042796833134Illumina Single End PCR Primer 1 (97% over 34bp)
ATATGTACATATATGTACATATAGATCGGAAGAGCGTCGTGTAGGGAAAG15090.13102666205888125Illumina Single End PCR Primer 1 (100% over 28bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT13020.11305282571283193No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)