FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001643769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001643769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48762593
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16093163.300308496720016No Hit
CAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG10948622.2452907703247034Illumina PCR Primer Index 7 (100% over 30bp)
CAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG3928900.8057200731716625Illumina PCR Primer Index 7 (100% over 30bp)
TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATAT3441780.7058238268830371No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATA1079220.22132129027674965No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)