FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001648528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895427
Sequences flagged as poor quality0
Sequence length35-301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA43108448.14284134831762No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTAACGAGACTGGGTGCTTCGGC689327.69822665610932No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAAACTATTATCTTA490775.480848801744866No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAAACATCAAA343083.831468115212072No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGG249372.7849283079469345No Hit
TCCGTAGGTGAACCTGCGGACTGCATCGATGAAGAACGCAGC181902.031433048143511No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTAACGAGACTGGGTGCTTCGGA103001.1502891916370626No Hit
TCCGTAGGTGAACCTGCGGAGCATCGATGAAGAACGCAGC98021.0946732676142221No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC96191.0742360907142625No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCCAGCAGAA75410.8421680382655425No Hit
TCCGTAGGTGAACCTGCGGAGCCCCGCATCGATGAAGAACGCAGC72910.8142483976918274No Hit
TCCGTAGGTGAACCTGCGGGGCATCGATGAAGAACGCAGC54100.604181022015195No Hit
TCCGTAGGTGAACCTGCGGGGGCATCGATGAAGAACGCAGC48520.5418643842546629No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG47490.5303614923382922No Hit
TCCGTAGGTGAACCTGCAGAGGGATCATTACCGAGTTTACAACTCCCAAA47470.5301381352137026No Hit
TCCGTAGGTGAACCTGCGGAGGGCATCGATGAAGAACGCAGC45530.5084724941284996No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACGTATCCACGTTATCAA42470.4742988540662723No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTTACAACTCCCAAA34690.3874129326008709No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTCCAACTCCCAAA26340.2941613330846624No Hit
TCCGTAGGTGAACCTGCGGAGGTGAACCTGCATCGATGAAGAACGCAGC25200.2814299769830483No Hit
TCCGTAGGTGAACCTGCGGAGGCTGCATCGATGAAGAACGCAGC24960.2787496914879717No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCCAA21250.23731694487657845No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCACA20030.22369216027660546No Hit
TCCGTAGGTGAACCTGCAGATGGATCATTAACACAATTAATACACCTTAA16900.18873677027831415No Hit
TCCGTAGGTGAACCTGCGGAGGGAGCATTACCGAGTTTACAACTCCCAAA14630.16338573663738082No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGGTTACAACTCCCAAA14450.16137552251607332No Hit
TCCGTAGGTGAACCTGCGGACTGCACCGATGAAGAACGCAGC13750.1535580231554331No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGATTTTACAACTCCCAAA13190.14730402366692094No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACC13030.14551716667020315No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTTACAACTCCCAAA13020.1454054881079083No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAGTAAGGGTGCTCAGCG12910.14417702392266482No Hit
TCCGTAGGTGAACCTGCCCATTGTGGAGCGGCCGGTATGAAGAACGCAGC11580.1293237751374484No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTTCCAAA10340.1154756334128857No Hit
TCCGTAGGTGAACCTGCTGAGGGATCATTACCGAGTTTACAACTCCCAAA10090.11268366935551419No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCTCAAA10010.11179024085715529No Hit
TCCGTAGGTGAACCTGCCCATGCATCGATGAAGAACGCAGC9730.10866324111289921No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACCACTCCCAAA9720.10855156255060434No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAC9310.10397274149651506No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACACCTCCCAAA9240.10319099156045106No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGAGACTGGGTGCTTCGGC9100.10162749168832301No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGATTACAACTCCCAAA9040.10095742031455385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)