FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001648529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895427
Sequences flagged as poor quality0
Sequence length35-301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT50287756.160580371152534No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG525505.868708448594917No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG425604.75303961126926No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT264642.9554614725711867No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTCGTTGAAAGTTT228672.5537536839965735No Hit
GCTGCGTTCTTCATCGATGCAGTCCGCAGGTTCACCTACGGA203682.2746689568217175No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG108281.2092554725287488No Hit
GCTGCGTTCTTCATCGATGCTCCGCAGGTTCACCTACGGA97411.0878608753142356No Hit
GCTGCGTTCTTCATCGATGCGGGGCTCCGCAGGTTCACCTACGGA74310.8298833964131079No Hit
GCTGCGTTCTTCATCGATGCCCCGCAGGTTCACCTACGGA55690.6219379134200778No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT54260.6059678790119127No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT52020.580951881057864No Hit
GCTGCGTTCTTCATCGATGCCCCCGCAGGTTCACCTACGGA49170.5491234908038288No Hit
GCTGCGTTCTTCATCGATGCCCTCCGCAGGTTCACCTACGGA48850.5455497768103932No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGATATACATTGCTAAGAGTTG43770.48881706716460416No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG31790.3550261495353613No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGGTT31210.34854879292225943No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAACGTTT30480.34039625787473465No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT30230.3376042938173631No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGATAGTTT29300.32721818752394105No Hit
GCTGCGTTCTTCATCGATGCAGCCTCCGCAGGTTCACCTACGGA25800.28813069072073993No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTCCGCAGGTTCACCTACGGA25430.2839985839158301No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGACAGTTT23370.26099280008308884No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG21760.2430125515536163No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGCAAGTTT19650.21944837490940078No Hit
GCTGCGTTCTTCATCGTCACGGTAACCTAGATATCCACCGTTGAAAATTT17550.1959958768274801No Hit
GCTGCGTTCTTCACCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT14940.1668477720685215No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGCGATCCGTTGTTGAAAGTTT12780.14272520261283164No Hit
GCTGCGTTCTTCATACCGGCCGCTCCACAATGGGCAGGTTCACCTACGGA11840.13222741775711477No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGCTGTTGAAAGTTT10160.1134654192915782No Hit
GCTGCGTTCTTCATCGATGCATGGGCAGGTTCACCTACGGA10100.11279534791780906No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)