FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719406
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA24854234.548224507440864No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCATTTGCTCACGGGTGCAG565817.86496081489451No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCTCTTTGAGCGT469076.520240309366339No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCCAGCAGAA286263.979116104119232No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTGTAGGCTTTGC199332.7707580976527857No Hit
TCCGTAGGTGAACCTGCGGAGCATCGATGAAGAACGCAGC134121.864315838344412No Hit
TCCGTAGGTGAACCTGCGGACTGCATCGATGAAGAACGCAGC132661.8440213175870093No Hit
TCCGTAGGTGAACCTGCGGAGGGCATCGATGAAGAACGCAGC123991.7235052251440772No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGG121791.6929244404411417No Hit
TCCGTAGGTGAACCTGCGGAGCCCCGCATCGATGAAGAACGCAGC98801.373355240295466No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA79661.1073024133799272No Hit
TCCGTAGGTGAACCTGCGGGGGCATCGATGAAGAACGCAGC73001.0147260378701317No Hit
TCCGTAGGTGAACCTGCGGCATCGATGAAGAACGCAGC68130.9470313008231791No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTGCCTTCGGGCTAA67140.9332699477068582No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG63850.8875377742192865No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTTACAACTCCCAAA57080.793432359474344No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAAACTATTATCTTA42290.5878460841305189No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA36640.5093090688707071No Hit
TCCGTAGGTGAACCTGCGGGGCATCGATGAAGAACGCAGC33940.4717781058261955No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACCCCCAAA33030.4591287812445268No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACGACTCCCAAA32920.4575997420093799No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGGTTACAACTCCCAAA28930.4021373188436015No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAACATCAAAC27690.38490087655649247No Hit
TCCGTAGGTGAACCTGCAGAGGGATCATTACCGAGTTTACAACTCCCAAA26950.3746146126109596No Hit
TCCGTAGGTGAACCTGCGGAGCACGACGGCATCGATGAAGAACGCAGC24120.335276603197638No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGATTACAACTCCCAAA23190.32234927148230624No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTAGGGTTTCGTCCG22850.3176231502100344No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCTCTGGG20580.28606934053927824No Hit
TCCGTAGGTGAACCTGCGGAGGGATCGTTACCGAGTTTACAACTCCCAAA20140.27995318359869115No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTTACGACTCCCAAA17690.24589730972496754No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAGCTCCCAAA17170.23866912424972825No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACACCCAAA16520.22963389240567914No Hit
TCCGTAGGTGAACCTGCGGCGAGAGATTCTCTCGGCCCGTCGCATCGATGAAGAACGCAGC15290.21253645368540156No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCAGTTGCTCACGGGTGCAG15030.2089223609477819No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTCCAACTCCCAAA14560.20238919330670024No Hit
TCCGTAGGTGAACCTGCGGAGGGATTATTACCGAGTTTACAACTCCCAAA12660.17597851560871053No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC12260.1704183729354495No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCACA11580.16096613039090585No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC11530.16027111255674822No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCCAA11340.15763004478694922No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTCTAACC10160.1412276239008293No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGAGTTTACAACTCCCAAA9200.1278832814850029No Hit
TCCGTAGGTGAACCTGCGGAGGGAGCATTACCGAGTTTACAACTCCCAAA9170.12746627078450834No Hit
TCCGTAGGTGAACCTGCGGACTGCACCGATGAAGAACGCAGC9000.1251032101483724No Hit
TCCGTAGGTGAACCTGCGGAGCGTTCAACAGCATCGATGAAGAACGCAGC8610.11968207104194294No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTTACAACTCCCAAA8560.11898705320778531No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGATTTTACAACTCCCAAA8550.11884804964095379No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)