FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001648533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719406
Sequences flagged as poor quality0
Sequence length35-301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT26521036.86513595938872No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTG597438.304490093215792No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA512047.1175386360414015No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG300824.1815052974259315No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG243313.382095784577832No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT149382.076435281329319No Hit
GCTGCGTTCTTCATCGATGCAGTCCGCAGGTTCACCTACGGA146092.0307031078417475No Hit
GCTGCGTTCTTCATCGATGCTCCGCAGGTTCACCTACGGA134271.8664008918468848No Hit
GCTGCGTTCTTCATCGATGCCCTCCGCAGGTTCACCTACGGA130761.8176106398890197No Hit
GCTGCGTTCTTCATCGATGCGGGGCTCCGCAGGTTCACCTACGGA97811.359593887179145No Hit
GCTGCGTTCTTCATCGATGCCCCCGCAGGTTCACCTACGGA73151.0168110913726045No Hit
GCTGCGTTCTTCATCGATGCCGCAGGTTCACCTACGGA69850.9709399143182014No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG63200.8785025423752374No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG49660.6902917128853526No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG47750.6637420316205314No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG45640.6344122790190796No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATACGTTGTTGAAAGTTA42490.5906261554671494No Hit
GCTGCGTTCTTCATCGATGCCCCGCAGGTTCACCTACGGA35360.491516612316272No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGGGATCCGTTGTTGAAGGTTT35350.4913776087494405No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCATTGTTGAAAGTTA34700.4823423769053914No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT34180.4751141914301521No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT24800.34472884574218177No Hit
GCTGCGTTCTTCATCGATGCCGTCGTGCTCCGCAGGTTCACCTACGGA24580.3416707672718882No Hit
GCTGCGTTCTTCATCGATGCGAGAACCTAGAGATCCGTTGTCGAAAGTTG24460.3400027244699099No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGGGATCCGTTGTTGAAAGTTT21560.29969169008876767No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAACGTTT20420.28384528346997384No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGATAGTTT17890.24867738106159804No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGACCCGTTGTTGAAAGTTT17450.24256122412101094No Hit
GCTGCGTTCTTCATCGATGCGACGGGCCGAGAGAATCTCTCGCCGCAGGTTCACCTACGGA16790.2333869887101303No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT16710.23227496017547813No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGGTT16570.23032891023983676No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT16330.22699282463588014No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATTCGTTGTTGAAAGTTT16310.2267148175022171No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT15660.21767958565816797No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGACAGTTT15650.21754058209133648No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAATTT14820.20600328604431992No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGCAAGTTT14080.19571702209878705No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA13060.18153865828197152No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC11870.16499723382902004No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTCGTTGAAAGTTT9890.13747452759637813No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG8960.1245471958810463No Hit
GCTGCGTTCTTCATCGATGCTGTTGAACGCTCCGCAGGTTCACCTACGGA8790.12218413524491037No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT8640.1200990817424375No Hit
GCTGCGTTCTTCACCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT8450.11745801397263854No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGCGATCCGTTGTTGAAAGTTT8310.11551196403699719No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTCGAAAGTTG7260.10091658951968706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)