FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001648534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808949
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA28613535.371203870701365No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA13577816.784494448970207No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCTCTTTGAGCGT352094.352437545506578No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAATCGCGGCGGAGGGGC204152.5236448774891866No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCGCACAAACCAGG197932.446754987026376No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTATAAGTTCACCCAGGCTTGTA163652.0229952691702446No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG162302.0063069488929464No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA110971.3717799267939017No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCATAATTTATGCATGTTCTTC90431.1178702242044924No Hit
TCCGTAGGTGAACCTGCGGAGGGCATCGATGAAGAACGCAGC85951.0624897243213107No Hit
TCCGTAGGTGAACCTGCGGAGCATCGATGAAGAACGCAGC75390.9319499745966681No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG70350.8696469122280885No Hit
TCCGTAGGTGAACCTGCGGACTGCATCGATGAAGAACGCAGC66780.825515576383678No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTACAATAAAATGAACTAATAA64950.8028936311188962No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAGACCCACTGACGAGGA56460.6979426391527772No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAATGCCTGGGCTGTCAT56120.6937396547866429No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAATAATCAATAATCTTGGCT52940.654429389244563No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTTTACCA33220.41065629600877185No Hit
TCCGTAGGTGAACCTGCGGGGGCATCGATGAAGAACGCAGC32330.39965436634447904No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG28490.35218536644460896No Hit
TCCGTAGGTGAACCTGCGGCATCGATGAAGAACGCAGC27330.33784577272485655No Hit
TCCGTAGGTGAACCTGCGGAGCCCCGCATCGATGAAGAACGCAGC27040.33426087429491846No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGCTTTGGGCAGGCCA21370.26416992913026655No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTTGGCAGGCCA21360.2640463119430273No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCCGGCCA19350.23919925730793906No Hit
TCCGTAGGTGAACCTGCCGAAGGATCATTAGTGAAGATTTGGGCAGGCCA16320.20174324957444784No Hit
TCCGTAGGTGAACCTGCGGAGGCTGCATCGATGAAGAACGCAGC15720.19432621834009312No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCATAATTTTGTAATGTTCTTC15410.19049408553567654No Hit
TCCGTAGGTGAACCTGCAGAGGGATCATTACCGAGTTTACAACTCCCAAA14070.1739293824456177No Hit
TCCGTAGGTGAACCTGCAGAAGGAGCATTAGTGAAGATTTGGGCAGGCCA10730.1326412419077099No Hit
TCCGTAGGTGAACCTGCGGGGCATCGATGAAGAACGCAGC9720.12015590599654614No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCC8820.10903035914501409No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)