FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001648535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808949
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG31613139.07922501912976No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT14105117.436327877282746No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA391844.843815864782576No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT344744.261578912885732No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGTTGAAAGTTG218102.6960908536879336No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG202552.5038661275309075No Hit
GCTGCGTTCTTCATCGGTATAGGAGCCTAGATATCCATCGTTGAAAATTT92791.1470438803929544No Hit
GCTGCGTTCTTCATCGATGCCCTCCGCAGGTTCACCTACGGA90771.1220732085706269No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG85791.0605118493254828No Hit
GCTGCGTTCTTCATCGATGCTCCGCAGGTTCACCTACGGA75660.9352876386521276No Hit
GCTGCGTTCTTCATCGATGCAGTCCGCAGGTTCACCTACGGA72550.8968426934207225No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA66610.823414084200611No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG62110.7677863499429507No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTAAAAGTTG59080.7303303422094595No Hit
GCTGCGTTCTTCATCGATGTGGGAACCTAGACATCCATCGTTGAAAATTT48640.6012739987316876No Hit
GCTGCGTTCTTCATCGATGCCCCCGCAGGTTCACCTACGGA32630.40336288196165637No Hit
GCTGCGTTCTTCATCGATGCCGCAGGTTCACCTACGGA28250.34921855395086715No Hit
GCTGCGTTCTTCATCGATGCGGGGCTCCGCAGGTTCACCTACGGA27620.34143067115479464No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT25540.31571829620903175No Hit
GCTGCGTTCTTCATCGTTGTGGGAACCTAGACATCCATCGTTGAAAATTT21580.2667658900622907No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT21540.26627142131333376No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG20030.24760522604020774No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTT18980.23462542138008702No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG18570.22955711670327797No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT16820.20792410893641006No Hit
GCTGCGTTCTTCATCGATGCAGCCTCCGCAGGTTCACCTACGGA16110.19914728864242368No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG15690.1939553667783754No Hit
GCTGCGTTCTTCATCGATGGCAGAACCAAGAGATCCGTTGTCGAAAGTTG15470.191235788659112No Hit
GCTGCGTTCTTCATCGTTATTGGAGCCTAGATATCCATCGTTGAAAATTT15280.18888706210156636No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG14550.17986300743310146No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG12930.15983702310034378No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTTGAAAGTTG11010.13610252315040874No Hit
GCTGCGTTCTTCATCGATGCCCCGCAGGTTCACCTACGGA10770.13313571065666688No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGATAGTTT10270.1269548512947046No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA9820.12139207786893857No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGCCGAAAGTTG9780.1208976091199816No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAACGTTT9780.1208976091199816No Hit
GCTGCGTTCTTCACCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG9610.11879611693691443No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT9530.11780717943900049No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGATAGTTG9210.11385142944734465No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGACAGTTG9180.1134805778856269No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGCAAGTTG8300.10260226540857334No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)