FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773961
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA27484835.51186687701318No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG691098.92926129352771No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC424485.484514077582721No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAATTGCCTGGGGTTTGA383544.95554685572012No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA316824.093487914765731No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA258323.3376358757094993No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTGCCTTCGGGCTAA181432.3441749648884116No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATAGTGCCCTCTGATGCAAA177902.298565431591514No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAACCTGCCCAGCAGAAC156182.0179311360649956No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACCCCCAAA143121.8491887834141514No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTTTACCA136321.7613290592161621No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAATGCCTGGGCTGTCAT131941.704737060394516No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCCAGCAGAA122041.5768236384003844No Hit
TCCGTAGGTGAACCTGCGGAGCCCCGCATCGATGAAGAACGCAGC96071.2412770152501222No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCATAATTTATGCATGTTCTTC43200.5581676596107555No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG32530.4203054159059695No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCCTGGGGTTTGA30450.3934306767395256No Hit
TCCGTAGGTGAACCTGCAGAGGGATCATTACCGAGTTTACAACTCCCAAA28090.3629381842237529No Hit
TCCGTAGGTGAACCTGCGGGGGAGTCTGTTCGGGAGGGGCCCGCCCCTCT19150.24742848799874928No Hit
TCCGTAGGTGAACCTGCGGACTGCATCGATGAAGAACGCAGC18890.24406914560294382No Hit
TCCGTAGGTGAACCTGCGGGGCATCGATGAAGAACGCAGC17650.22804766648448693No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTTACAACTCCCAAA17560.22688481719363118No Hit
TCCGTAGGTGAACCTGCGGAGGGCATCGATGAAGAACGCAGC15300.19768437944547593No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTCCAACTCCCAAA15100.19510026991024096No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCACA12700.1640909554874212No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCCAA11140.14393490111258836No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGGTTACAACTCCCAAA10520.13592416155335993No Hit
TCCGTAGGTGAACCTGCGGAGGGAGCATTACCGAGTTTACAACTCCCAAA10050.1298515041455577No Hit
TCCGTAGGTGAACCTGCGGAGCATCGATGAAGAACGCAGC9260.11964427148137954No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTTACAACTCCCAAA9070.11718936742290632No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGATTTTACAACTCCCAAA8300.10724054571225165No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC8060.10413961426996966No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)