FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001648537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773961
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT32139941.52651102574936No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG12835716.584427380707815No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA718509.283413505331664No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG276383.5709809667412182No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG209862.7115061353220637No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA198692.5671836177791905No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTAAAAGTTT193162.4957329891299436No Hit
GCTGCGTTCTTCATCGATGCGGGGCTCCGCAGGTTCACCTACGGA96201.242956686448025No Hit
GCTGCGTTCTTCATCGGTATAGGAGCCTAGATATCCATCGTTGAAAATTT45610.5893061795103371No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT39150.5058394415222472No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAACGTTT22330.2885158296089855No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA21490.27766256956099855No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG21370.27611210383985757No Hit
GCTGCGTTCTTCATCGATGCAGTCCGCAGGTTCACCTACGGA20650.2668093095130116No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT20380.26332076164044443No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGGTT20130.26009062472140065No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGATAGTTT19640.253759556360075No Hit
GCTGCGTTCTTCATCGATGCCCCGCAGGTTCACCTACGGA17950.2319238307873394No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGACAGTTT16520.21344744761040932No Hit
GCTGCGTTCTTCATCGATGCCCTCCGCAGGTTCACCTACGGA16460.21267221474983883No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG15660.2023357766088989No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT15500.20026848898071092No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGCAAGTTT15180.19613391372433495No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG12940.1671918869297032No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTCGTTGAAAGTTT12000.15504657211409875No Hit
GCTGCGTTCTTCATCAACGCAGAAGGCCAGTGATCCACCGCTAAAAGTCA11640.1503951749506758No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT11540.14910312018305832No Hit
GCTGCGTTCTTCACCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT9710.12545851793565826No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGCGATCCGTTGTTGAAAGTTT9230.1192566550510943No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTT9200.11886903862080905No Hit
GCTGCGTTCTTCATCGATGCTCCGCAGGTTCACCTACGGA9090.11744777837642982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)