FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750725
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA23448831.234872956142397No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATATAGGACGTCCAAC532447.092344067401511No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG488336.504778713909887No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG397945.3007426154717106No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCCAGCAGAA316004.209264377768158No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGAATTTAGATTGAACCAT280883.7414499317326584No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA256743.4198941023677114No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAACATCAAAC213862.8487129108528424No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCTCTGGG189112.5190316027839756No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGCGTAACTTGGTCTTGGTAC177752.367711212494589No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACACAATAACCAGGCGGGCTG167612.2326417796130404No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAGTAAGGGTGCTCAGCG159992.131139898098505No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATATAGAAAATCTCTTT95001.2654434047087815No Hit
TCCGTAGGTGAACCTGCGGACTGCATCGATGAAGAACGCAGC63370.8441173532252157No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC43210.5755769422891206No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGTATTATTTTTTTTCTTC31330.41732991441606443No Hit
TCCGTAGGTGAACCTGCAGAGGGATCATTACCGAGTTTACAACTCCCAAA26350.35099403909554094No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG26090.3475307203037064No Hit
TCCGTAGGTGAACCTGCGGGGGCATCGATGAAGAACGCAGC18530.24682806620267075No Hit
TCCGTAGGTGAACCTGCGGAGCCCCGCATCGATGAAGAACGCAGC18100.24110026973925205No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTTACAACTCCCAAA15250.2031369675979886No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTCCAACTCCCAAA13690.18235705484698125No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA12870.17143428019581072No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCACA12040.16037830097572348No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCCAA10290.13706750141529855No Hit
TCCGTAGGTGAACCTGCTGAGGGATCATTACCGAGTTTACAACTCCCAAA10130.13493622831263113No Hit
TCCGTAGGTGAACCTGGGAGGCAGAGATTGCAGGGAGCCAAGATGGCTCC10020.13347097805454727No Hit
TCCGTAGGTGAACCTGCAAAAATTGAGCAATGACCGCATCAGGGTGGGGG9670.12880881814246228No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGGTTACAACTCCCAAA9440.12574511305737787No Hit
TCCGTAGGTGAACCTGCGGAGGGAGCATTACCGAGTTTACAACTCCCAAA8620.11482233840620733No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTTACAACTCCCAAA7970.10616404142662095No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)