FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001648539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750725
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT23941531.891171867195045No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT8977211.958040560791234No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG522976.9661993406373846No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA509656.788770854840322No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT420525.601518532085651No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG278553.710413267175064No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT234013.1171201172200207No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT200942.676612607812448No Hit
GCTGCGTTCTTCATCGATGCGGGAACCAAGATATCCATTGCTAAGAGTTG190382.535948583036398No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG188702.51357021545839No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCTAGAGATCCGTTGTTGAAAGTTT101121.3469646008858103No Hit
GCTGCGTTCTTCATCGATGCAGTCCGCAGGTTCACCTACGGA69720.9287022544873289No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG42990.5726464417729528No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT27510.36644576908987975No Hit
GCTGCGTTCTTCATCGATGCCCCCGCAGGTTCACCTACGGA18480.2461620433580872No Hit
GCTGCGTTCTTCATCGATGCGGGGCTCCGCAGGTTCACCTACGGA18000.2397682240500849No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT16770.22338406207332911No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAACGTTT15970.212727696559992No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGGTT15170.20207133104665492No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGATAGTTT14370.1914149655333178No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGCAAGTTT12320.16410802890539145No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGACAGTTT12040.16037830097572348No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG11690.15571614106363849No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA11540.15371807252988778No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG11150.14852309434213595No Hit
GCTGCGTTCTTCATCTCCTTCTTTTTCTTTTTTGTGAGACTGGGTCTCAC9560.12734356788437845No Hit
GCTGCGTTCTTCATCCCGGTTTCAACTCCACTATAGGAACCCTGGAGGAC9320.1241466582303773No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTCGTTGAAAGTTT8240.10976056478737221No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG7910.10536481401312064No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGCGATCCGTTGTTGAAAGTTT7770.10349995004828665No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)