FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840798
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAATGAAAAGTGCTTAAC40564148.244762713517396No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA522956.219686535886146No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTATTGGGTCTTAGTG473395.630246503916517No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA168992.009876331770532No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG149301.7756940430400645No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGG139211.6556890002117037No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCTTCAGTGGTTTTCCACTG134531.6000275928344263No Hit
TCCGTAGGTGAACCTGCGGAGCATCGATGAAGAACGCAGC116221.3822582832023862No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG101781.2105166758246333No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCCGGCTCCGG81430.9684847014383954No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTTTACCA80800.9609918196760696No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGCGAAGTCTGGAATGCGTGT78130.9292362731595459No Hit
TCCGTAGGTGAACCTGCCGATCGGCGAGACGCTGGCGCTGCGCGTCGCCG78070.9285226653726578No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAGATAGGGTCCTTCGGG52390.6230985325845209No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCACACCATCCAAACCTTGC51020.606804488117241No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG43110.5127271948791505No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGATATAAGAGTTGGGC39090.46491547315764314No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTACAGAATGAAAAGTGCTTAAC39040.4643208000019029No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAATGAACAGTGCTTAAC35960.42768893360831023No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTCTGAGATTACTATCACATTT34400.40913513114921773No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAATGCAAAGTGCTTAAC29380.3494299463129075No Hit
TCCGTAGGTGAACCTGCGGCAGGATCATTACAGAATGAAAAGTGCTTAAC27860.3313518823784072No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAATGACAAGTGCTTAAC23060.2742632594273535No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTTTTAACTCTCCAA21850.25987216905844207No Hit
TCCGTAGGTGAACCTGCGGCTTCCTGGGAGAGAGGAGAAAAGTCAAAGGC20840.24785977131249123No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAATGAAAAGTGCTTCAC20730.24655149036986293No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAATTAAAAGTGCTTAAC16020.19053327909914153No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC15280.1817321163941874No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAATGAAAAGTGCTCAAC15110.17971022766467096No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAATGAAAAGTGCTTAAC14510.17257414979578925No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTAACAGAGAAGAGCAAAGCCAC14400.17126586885316092No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAATGTAAAGTGCTTAAC13050.15520969364817708No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC13040.15509075901702907No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCGATTTTCCAGCCTTCTGG12230.14545705389403876No Hit
TCCGTAGGTGAACCTGCGGAGCGTTCTGCATCGATGAAGAACGCAGC12080.14367303442681834No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAATGAAACGTGCTTAAC11740.13962925696778536No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAATGAAAAGTGCCTAAC11100.13201744057431156No Hit
TCCGTAGGTGAACCTGCGGGGCAAAGATTCGATGATTGCATGAATTCCAC9920.11798315409884419No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)