FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001648541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840798
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT45215953.77736388526139No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT743528.84302769511821No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG520486.190309681992583No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG185182.2024314995991903No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT169492.0158230633279337No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA152711.816250752261542No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG149501.778072735663025No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT146341.7404893922202478No Hit
GCTGCGTTCTTCATCGATGCTCCGCAGGTTCACCTACGGA115981.3794038520548337No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA92331.0981234493897465No Hit
GCTGCGTTCTTCATCGATGCGCGTATCGTTGTAGACGTTGCGGGTATAGC75090.8930801452905455No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCATTGCTGAAAGTTA42300.5030934897561602No Hit
GCTGCGTTCTTCATCGATGCGAGAACCGAGAGATCCGTCGTTGAAGCTTG35580.4231694176246852No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTAGAAAGTTG34550.4109191506164382No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT25160.2992395319684395No Hit
GCTGCGTTCTTCATCGATGTGGGACCCAAGACATCCATTGCCAAAAGTTG24030.2857999186487123No Hit
GCTGCGTTCTTCATCGTTGTGGGACCCAAGACATCCATTGCCAAAAGTTG23900.2842537684437879No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGGTT21530.2560662608617052No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG21390.2544011760256328No Hit
GCTGCGTTCTTCATCGATGCTCACACGTGTACTGAGCAGTGAGAGGGGAA20480.24357812459116218No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG19830.23584737356654034No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGATAGTTT17560.20884921229593792No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGCTGAAAGTTT17230.20492436946805298No Hit
GCTGCGTTCTTCATCGATTCGAGAACCAAGAGATCCGTTGTTGAAAGTTT15490.184229743648296No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA14790.17590431946793406No Hit
GCTGCGTTCTTCATCGATGCATGCGCCACAGGGGAACACAGTCAGCCAAG13870.16496233340231542No Hit
GCTGCGTTCTTCACCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT12620.15009550450881184No Hit
GCTGCGTTCTTCATCGATGCAGAACGCTCCGCAGGTTCACCTACGGA12010.14284049200878213No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT11690.13903458381204523No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAACGTTT10590.1259517743857621No Hit
GCTGCGTTCTTCATCGATGCGTTCTGGGCCGCACGGGTACATGGTGGAAT10020.11917250041032447No Hit
GCTGCGTTCTTCATCGACCCCCGAGCCAAATGATCCACTGTATAGTGTTG9960.11845889262343631No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGACAGTTT9180.10918199139389008No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT8770.10430567151682092No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCAAAAGTTG8550.1016891096315643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)