FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690545
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG19726828.567001426409576No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAAACATCAAA16098123.31216647720279No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCTTCAGTGGTTTTCCACTG11351216.438030830720663No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG485857.035747127269041No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTCTAACC67270.9741580925211246No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTTACTATACTTTTG21150.30627982245907215No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTATTGATTTGACTATACTTTTG20630.29874953840806895No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTGTCGATGCCTAAAACATCAAA18220.26384956809476573No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG16790.24314128695450696No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCTGCAGTGGTTTTCCACTG16440.2380728265355625No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG15930.2306873556393863No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAAACATCCAA13690.19824920895814174No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTATCTTCAGTGGTTTTCCACTG13240.19173261699092747No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAATGAAAAGTGCTTAAC12710.18405751978509727No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAAACATCACA11470.1661006885865512No Hit
TCCGTAGGTGAACCTTCGGAAGGATCATTATCTTCAGTGGTTTTCCACTG10810.15654302036797021No Hit
TCCGTAGGTGAACCTGCGTGCCTGTGGCTCACTCCAGGGGTGTGTTGCGT10700.1549500756648734No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGTCTATACTTTTG9120.13206959720220984No Hit
TCCGTAGGTGAACCTGCGGAAGGAGCATTGTCGATGCCTAAAACATCAAA8530.12352562106741777No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTCAAACATCAAA8210.11889160011295426No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCTTCAGTTGTTTTCCACTG8160.11816753433881934No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAAACATCAAC7450.10788580034610344No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAAACCTCAAA7320.10600322933335263No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCTTCATTGGTTTTCCACTG7110.10296215308198597No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAACGGAGCCCGGAGGGCGAGG6940.10050032944992723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)