FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690545
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA14965021.671288619858228No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG14427220.892483473198705No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG12719418.419364415063463No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA648099.385195751182037No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG273223.95658501618287No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCATTGCCGAGAGTCG244893.546329348558023No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT73321.0617700511914503No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT24820.3594262502805755No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG16970.24574792374139268No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT16780.24299647379967998No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAAAGTTA15890.23010810302007834No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG15850.2295288504007704No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAGAGTTA14020.20302804306743225No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGGCG13900.20129028520950842No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGCGTCG13200.1911533643716195No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGAGATCCGTTGCCGAGAGTCG13110.18985004597817665No Hit
GCTGCGTTCTTCATCGATGCCCGAGCCGAGTGATCCACCGCTAAGAGTCG12680.1836230803206163No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA12060.1746446647213433No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTACGAGTCG11590.16783844644447501No Hit
GCTGCGTTCTTCATCGATGCCAAAGGGTTCAGGATTTCCTCCGCCCTCCC11010.15943928346450992No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTCAGAGTCG9310.13482104714392257No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCACCGCTAAGAGTCG9010.13047665249911303No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTG8750.12671151047361143No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTCGATAGTTA8430.1220774895191479No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGCGTGATCCACCGCTAAGAGTCG7900.11440239231331774No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTTGATAGTTA6990.10122439522406215No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTT6960.1007899557595812No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)