FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001648544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889358
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG13064614.689922393456852No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC12284813.813110131128298No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAACATCAAAC12040013.53785539681433No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAGTAAGGGTGCTCAGCG11690513.144875292064611No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCTTCAGTGGTTTTCCACTG9795711.014349676957986No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTCTGAGATTACTATCACATTT595536.696178591748205No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA345193.8813391232776904No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG116771.3129695803039945No Hit
TCCGTAGGTGAACCTGGCATCGATGAAGAACGCAGC24560.2761542595895017No Hit
TCCGTAGGTGAACCTGCATGAGGCAGCGGTCCCTCAGAGAGTCTGTTTCT13780.15494322871104774No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTGTCGATGCCTAAACATCAAAC13750.15460590673272182No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC13360.1502207210144846No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTATTGATTTGACTATACTTTTG11980.13470391001149143No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTTACTATACTTTTG11940.13425414737372351No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAAAGAGTAAGGGTGCTCAGCG11800.13267997814153581No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCTGCAGTGGTTTTCCACTG11740.13200533418488392No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTATCTTCAGTGGTTTTCCACTG10130.11390238801472523No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAGTACGGGTGCTCAGCG10120.11378994735528325No Hit
TCCGTAGGTGAACCTGCCCTTGCATTCCTGAGTTAAACCCTGCTTTGCCT9590.10783059240485834No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG9520.10704350778876447No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGAGTAAGGGTGCTCAGCG9490.10670618581043856No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGCTTCAAGGGCCAGC9490.10670618581043856No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG9320.1047946945999249No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)