FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001648545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889358
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG16565718.626582321179995No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA13502015.18173783785607No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT12787814.378686648121452No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG12328413.862134258645002No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG10638011.961437351437779No Hit
GCTGCGTTCTTCATCGATGCGAGAACCGAGAGATCCGTCGTTGAAGCTTG646237.266252735119042No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG63790.7172589665803872No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG47800.537466352132662No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA38160.42907355643059375No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT26030.29268303652747263No Hit
GCTGCGTTCTTCATCGATGCCAGGTTCACCTACGGA24890.27986480135108693No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT24560.2761542595895017No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG18900.21251284634534123No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG18190.20452955952496069No Hit
GCTGCGTTCTTCATCCCTCTGTGTAGCACATGGACTGCCCACACTCAGGG13590.15280685618165013No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAAAGTTA13340.14999583969560065No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGAGATCCGTTGCCGAGAGTCG12150.13661540122200508No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTT11780.13245509682265183No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGGCG11330.12739526714776278No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAGAGTTA11090.12469669132115525No Hit
GCTGCGTTCTTCATCGATGCTAAAATAAAATACATGAAGCACTGTATTAA9730.10940476163704604No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG9610.10805547372374229No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)