FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001648548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840399
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCTCTGGG21209925.23789295322817No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA18945822.543815497162658No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGAAATTTATGACTGGAGT12093014.38959351450918No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACTAATCCAAACCTACTC625457.44229824166854No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGAGAAATCTATATGAATGAA394264.691343040627131No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA31950.38017655899162184No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTCGGGCCCTCTGGG30860.36720652927954456No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTACCGAGTGCGGGCCCTCTGGG22010.2618994073053395No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCTGGCCCTCTGGG18490.2200145407122093No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGCTTTGGGCAGGCCA15320.18229436255873696No Hit
TCCGTAGGTGAACCTGCCCATTGTGGAGCGGCATCGATGAAGAACGCAGC13880.16515964440700193No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTTGGCAGGCCA13650.16242284914665533No Hit
TCCGTAGGTGAACCTGCGGACTGCATCGATGAAGAACGCAGC13090.15575934764320282No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAATGAAATTTATGACTGGAGT12830.1526655790880284No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG12220.1454071220931962No Hit
TCCGTAGGTGAACCTGCCGAAGGATCATTAGTGAAGATTTGGGCAGGCCA11650.13862462949146773No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGTGCCCTCTGGG11320.1346979232483618No Hit
TCCGTAGGTGAACCTGCGGAAGGAGCATTACCGAGTGCGGGCCCTCTGGG9700.11542136532765984No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGAAATTTATGACTGGAGT9320.11089970359317419No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGAGAAATCTATATGACTGAA9190.109352819315587No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCCGGCCA9160.1089958460207592No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGAAATTTATGACTGGCGT9070.10792492613627575No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCTCCGGG9010.10721097954662012No Hit
TCCGTAGGTGAACCTGCAGAAGGAGCATTAGTGAAGATTTGGGCAGGCCA8650.10292730000868636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)