FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001648549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840399
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT23628028.115216700638623No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG20073923.88615407681351No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG12740415.159941884747601No Hit
GCTGCGTTCTTCATCGTTGTAGGAACCTAGATATCCATCGTTTAGGATTT479695.707883993198469No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT454425.4071934878551735No Hit
GCTGCGTTCTTCATCGATGTAGGAACCTAGATATCCATCGTTTAGGATTT129151.536770034233739No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG65530.7797486670022216No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT37520.4464546007313193No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG27690.3294863511260722No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT19810.23572136568463312No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT17620.2096623151622027No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCGAAAGTTG17230.20502166232944113No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTCGAAAGTTT16680.19847715192426457No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTG15800.188005935275982No Hit
GCTGCGTTCTTCATCGATGCCGCTCCACAATGGGCAGGTTCACCTACGGA15000.17848664741390696No Hit
GCTGCGTTCTTCATCGATGCAGTCCGCAGGTTCACCTACGGA14890.17717774533287164No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG12680.15088071261388936No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTT12480.1485008906483706No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG10780.12827240394146114No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGGTT10090.12006201816042142No Hit
GCTGCGTTCTTCATCGATGGCAGAACCAAGAGATCCGTTGTCGAAAGTTG9960.11851513388283423No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG9350.11125667688800202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)