FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001648551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765550
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT20346526.577623930507478No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG13952218.22506694533342No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT10198713.322056038142513No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA8554511.174319116974724No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTA613358.01188687871465No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT161942.1153419110443474No Hit
GCTGCGTTCTTCATCGATGTGCGAGCCTAGACATCCACTGCTGAAAGTTG150481.9656456142642542No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG65430.8546796420873881No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG38960.5089151590359873No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT29340.3832538697668343No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT28260.36914636535823914No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA22780.2975638429887009No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTCGAAAGTTT16480.21527006727189604No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCGAAAGTTT13290.17360067925021228No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT12540.16380380118868787No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTT9840.12853504016720005No Hit
GCTGCGTTCTTCATCGATGCTCACAGGGCCTGGGGGGCCTTCCGCAGGTTCACCTACGGA9460.1235712886160277No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAAAGTTA9420.1230487884527464No Hit
GCTGCGTTCTTCATCAACGCAGAAGGCCAGTGATCCACCGCTAAAAGTCA9080.11860753706485533No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT8630.1127294102279407No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG8530.11142315981973745No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG7940.10371628241133825No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTA7710.10071190647247077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)