FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001648553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757754
Sequences flagged as poor quality0
Sequence length35-301
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCAAGAGCCTAGATATCCGTTGCCGAGAGTCA26403734.84468574233854No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA11506415.184875302538819No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT727819.604832175085846No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG684359.0312951168849No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT154862.0436711650482877No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCTAGATATCCGTTGCCGAGAGTCA56890.7507713585147686No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG46570.6145794017583542No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA36980.4880211783771514No Hit
GCTGCGTTCTTCATCGATGCCCGAGCCGAGTGATCCACCGCTAAGAGTCG33330.43985251150109406No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGCGTCG33030.4358934429907331No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTACGAGTCG30000.39590685103608825No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCTAGATATCCGTTGCCGAGAGTCT27820.3671376198607992No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCACCGCTAAGAGTCG23580.31118278491436535No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTCAGAGTCG23380.3085434059074581No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGCGTGATCCACCGCTAAGAGTCG21780.28742837385220005No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA20270.2675010623500503No Hit
GCTGCGTTCTTCATCGATGCCGCAGGTTCACCTACGGA19430.2564156705210398No Hit
GCTGCGTTCTTCATCGATGCACGAGCCTAGATATCCGTTGCCGAGAGTCA18290.24137121018166846No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCCAAGAGTCG16220.2140536374601784No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCTAGATATCCGTTGCCGCGAGTCA15400.20323218353185862No Hit
GCTGCGTTCTTCATCGATGCAAGAACCTAGATATCCGTTGCCGAGAGTCA15020.19821736341873483No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCTAGATATCCGTTGCCGAGAGTCA13370.17644248661175No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGCGATCCACCGCTAAGAGTCG12430.1640374052792859No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGGCA12330.16271771577583224No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCTAGATATCCGTTGCTGAGAGTCA11750.15506351665580123No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAAAGTTA11400.15044460339371352No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11370.15004869654267744No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCTAGATATCCGTTGCCGAGAGTCT11340.14965278969164134No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAGAGTTA10770.14213055952195566No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCTAGATATCCGTTGCCGAGCGTCA10400.13724770835917727No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCACCGCTAAGAGGCG10050.13262879509708955No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGCTCCACCGCTAAGAGTCG9810.12946154028880086No Hit
GCTGCGTTCTTCACCGATGCAAGAGCCTAGATATCCGTTGCCGAGAGTCA9130.12048765166531618No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCAAGAGATCCGTTGCCGAGAGTCA9020.1190359932115172No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCTAGATATCCGTTGCCGAGAGTCG8670.11441707994942948No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGAGATCCGTTGCCGAGAGTCA8610.11362526624735733No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCTAGATATCCGTTGCCGATAGTCA8250.10887438403492425No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCTAGATATCCGTTGCCGAGAGGCA8060.10636697397836237No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT7670.10122018491489322No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)