FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712073
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAGGGATCATTACACAAATATGAAGGCGGGCT8805912.366569157937459No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG8273711.619173876835662No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTAATGAGAACGTGCCCTCACGG7205610.11918721816443No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG691189.706589071626084No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA547307.686009720913447No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATATAGGACGTCCAAC438536.158497794467702No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAACATCAAAC403475.666132545399138No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC374305.256483534693775No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA122301.717520535113675No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN82281.1554995063708358No Hit
TCCGTAGGTGAACCTGCGGTTGGTGGCCGACAAACTGCGGCTGCTGGGCC56270.7902279681998896No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGATCAT29620.41596858749032756No Hit
TCCGTAGGTGAACCTGCGGCATCGATGAAGAACGCAGC25230.35431760507700755No Hit
TCCGTAGGTGAACCTGCGGATGTAGATGAAGAACGCAGC21120.2965988037743321No Hit
TCCGTAGGTGAACCTGCGGATGTGAAGAACGCAGC19950.2801679041334245No Hit
TCCGTAGGTGAACCTGCAAAAATTGAGCAATGACCGCATCAGGGTGGGGG17280.24267174854263537No Hit
TCCGTAGGTGAACCTGCGGATGGCATCGATGAAGAACGCAGC15830.22230866779108321No Hit
TCCGTAGGTGAACCTGCGGATGTAATCGATGAAGAACGCAGC13800.1938003547389102No Hit
TCCGTAGGTGAACCTGCGGATGTAGAAGATGAAGAACGCAGC13410.188323388191941No Hit
TCCGTAGGTGAACCTGCGGATGTAGCATCGATGAAGAACGCAGC12960.18200381140697652No Hit
TCCGTAGGTGAACCTGCGGATGTATGAAGAACGCAGC12460.1749820594236827No Hit
TCCGTAGGTGAACCTGCGGATGTCGATGAAGAACGCAGC11720.1645898664884078No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAACGGAGCCCGGAGGGCGAGG10820.15195071291847886No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC10750.15096766764081773No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGCGG10730.15068679756148598No Hit
TCCGTAGGTGAACCTGCAGAGGGATCATTACACAAATATGAAGGCGGGCT10120.14212026014186746No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGCGGGCGAGG8560.12021239395399066No Hit
TCCGTAGGTGAACCTGCGGATTCGATGAAGAACGCAGC8340.11712282308134138No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACACAAATATGAAGGCGGGCT8150.11445455732768972No Hit
TCCGTAGGTGAACCTGCAGAGGGATCATTAATGAGAACGTGCCCTCACGG8100.11375238212936033No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC7570.10630932502706884No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGAGAACGTGCCCTCACGG7480.10504540967007595No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTTACTATACTTTTG7450.10462410455107833No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC7330.1029388840750878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)