FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001648555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712073
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG9729913.664188924450164No Hit
GCTGCGTTCTTCATCGATGCCAGAACCTAGAGATCCGTTGTTGAAAGTTT7584010.650593408260107No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA7163010.059361891266766No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG610838.578193527910761No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG518997.288438123619348No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT485886.8234577072856295No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG475036.671085689248153No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG415965.841535909941817No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN78331.100027665702814No Hit
GCTGCGTTCTTCATCCGGGGCCTGGCGTTGCACGGTGACGTCGTCGTCCA44890.6304128930601217No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG35730.5017743967261784No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTG28420.3991163827304223No Hit
GCTGCGTTCTTCATCGATGCCGCAGGTTCACCTACGGA26030.3655524082502777No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT24310.3413975814277469No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA21980.30867621718559757No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGCGTCG21970.3085357821459317No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTACGAGTCG21970.3085357821459317No Hit
GCTGCGTTCTTCATCTACATCCGCAGGTTCACCTACGGA21940.30811447702693406No Hit
GCTGCGTTCTTCATCGATGCCCGAGCCGAGTGATCCACCGCTAAGAGTCG21670.30432273095595536No Hit
GCTGCGTTCTTCACATCCGCAGGTTCACCTACGGA19380.27216310687246953No Hit
GCTGCGTTCTTCATCCCGGTTTCAACTCCACTATAGGAACCCTGGAGGAC17050.23944174263032023No Hit
GCTGCGTTCTTCATCGATGCCATCCGCAGGTTCACCTACGGA16880.2370543469560003No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTCAGAGTCG16200.22750476425872065No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG16200.22750476425872065No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCACCGCTAAGAGTCG14730.20686081342783674No Hit
GCTGCGTTCTTCATCGATTACATCCGCAGGTTCACCTACGGA14640.20559689807084386No Hit
GCTGCGTTCTTCATCTTCTACATCCGCAGGTTCACCTACGGA14280.20054123664287227No Hit
GCTGCGTTCTTCATCGATGCTACATCCGCAGGTTCACCTACGGA13690.19225556930258555No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT13240.18593599251762108No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGCGTGATCCACCGCTAAGAGTCG13220.18565512243828933No Hit
GCTGCGTTCTTCATACATCCGCAGGTTCACCTACGGA12460.1749820594236827No Hit
GCTGCGTTCTTCATCGACATCCGCAGGTTCACCTACGGA12030.16894335271804997No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC11060.15532115387045992No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT10890.15293375819614No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCCAAGAGTCG10860.15251245307714237No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG10530.14787809676816843No Hit
GCTGCGTTCTTCTTCCGGGGCCTGGCGTTGCACGGTGACGTCGTCGTCCA10020.1407159097452087No Hit
GCTGCGTTCTTCATCGATGCCAGAACCGAGAGATCCGTTGTTGAAAGTTT8760.1230210947473082No Hit
GCTGCGTTCTTCATCGAATCCGCAGGTTCACCTACGGA8600.12077413411265417No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGCGATCCACCGCTAAGAGTCG8340.11712282308134138No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAAAGTTA7600.10673063014606649No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA7410.10406236439241483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)