FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665392
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG26072439.18351888811407No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG615729.253492678000336No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC507017.61971890254166No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA406986.116394546372664No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACACAATAACAAGGCGGGCTG341145.126902637843557No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCAAGGCAGAA267064.0135739533988986No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG214833.228623127419626No Hit
TCCGTAGGTGAACCTGCAGATGGATCATTTCGATGAAAAACCTTTTTTCA75241.130762016976459No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTTACTATACTTTTG27270.40983360184673095No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTATTGATTTGACTATACTTTTG24620.37000745425253084No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG19780.2972683771370861No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG18230.27397383797821434No Hit
TCCGTAGGTGAACCTGCGCATCGATGAAGAACGCAGC17700.26600860845937435No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA13350.20063361146512132No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTTTTAACTCTCCAA11890.17869165845095825No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGTCTATACTTTTG11120.16711953254622838No Hit
TCCGTAGGTGAACCTGCAAAAATTGAGCAATGACCGCATCAGGGTGGGGG9100.13676148796498905No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC9030.1357094765191045No Hit
TCCGTAGGTGAACCTGCTGAGGGATCATTAACTGAGAAGAGCGAAGCCGC8490.12759395965085243No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAACGGAGCCCGGAGGGCGAGG7800.1172241325414192No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGCGG7040.10580229398610144No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)