FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665392
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA27294141.019579435881404No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG9613814.448325197778153No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG364365.4758698631783975No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG363765.466852622213673No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG268404.033712458220117No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT226703.4070142111717603No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGCCAAAAGTTG86221.2957775266309182No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA66891.0052720802173756No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG38450.5778548584894317No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAAAGTTA26380.39645802774905625No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT24050.3614410753360425No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAGAGTTA22810.3428054440089451No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG22550.33889797292423113No Hit
GCTGCGTTCTTCATCGATGCGCAGGTTCACCTACGGA18080.2717195277370332No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGCGTCG17910.2691646427970279No Hit
GCTGCGTTCTTCATCGATGCCCGAGCCGAGTGATCCACCGCTAAGAGTCG16610.24962728737345807No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTACGAGTCG15890.2388065982157886No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTCGATAGTTA15460.23234424219106933No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTT12560.18876091086156732No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTCAGAGTCG11890.17869165845095825No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA11590.17418303796859597No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCACCGCTAAGAGTCG10610.15945487772621253No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG10530.1582525789309159No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTTGATAGTTA10340.15539711929208647No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGCGTGATCCACCGCTAAGAGTCG10260.15419482049678987No Hit
GCTGCGTTCTTCATCCCGGTTTCAACTCCACTATAGGAACCCTGGAGGAC8690.13059970663909395No Hit
GCTGCGTTCTTCACCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA8040.120831028927309No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCCAAGAGTCG7980.11992930483083657No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT7480.11241493736023278No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTT7430.11166350061317237No Hit
GCTGCGTTCTTCATCAACGCAGAAGGCCAGTGATCCACCGCTAAAAGTCA7370.11076177651669994No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAGAGGTA7030.10565200663668936No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGGTA7010.1053514319378652No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)