FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001648558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649594
Sequences flagged as poor quality0
Sequence length35-301
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG30894647.559860466691504No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAAACTATTATCTTA17536126.99547717497391No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCACACCGATCCAACTTGAA109221.6813578943155265No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG73711.1347087565463967No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTTACTATACTTTTG34290.5278681761223164No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTATTGATTTGACTATACTTTTG30590.470909521947555No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG23750.36561298287853644No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTGCACATATCCAAACCTTAAA21180.32604980957336427No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG20090.30927009793809673No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGATAAAACTATTATCTTA18680.2875642324282552No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAACACTATTATCTTA15610.24030394369406122No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAAACTATTCTCTTA14920.22968192440201107No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGTCTATACTTTTG12850.19781586652586075No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAACCTATTATCTTA8430.12977336613330787No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATAAAACTATTATCTTA7440.11453307758384468No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTCTACTTTTG6920.106528077537662No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAATACTATTATCTTA6640.10221769289740976No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAAACTATCATCTTA6630.10206375058882933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)